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Chapter 1: Working Safely with Wild Poliovirus, Chapter 2: Biocontainment of Highly Pathogenic Avian Influenza Viruses, Chapter 3: Maximum Containment for Researchers Exposed to Biosafety Level 4 Agents, Chapter 4: Modular/Mobile BSL-2/3 Laboratories, Chapter 5: Facility Maintenance Operations (Skilled Trades) for Biological Containment Laboratories, Chapter 6: Construction and Commissioning Guidelines for Biosafety Level 4 (BSL-4) Facilities, Chapter 7: Safety and Health Considerations for Conducting Work with Biological Toxins, Chapter 8: Primary Containment Devices for Toxicological Research and Chemical Process Laboratories, Chapter 9: Toxicology Laboratories, Chapter 10: Medical and Infectious Waste Management